Catalog Number - 68004-P21-1503M

Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. The 300 Å pore structure is specifically designed to improve analyte accessibility for large biomolecules, making this stationary phase an excellent choice for proteins, peptides, enzymes, and other high-molecular-weight compounds. The moderate hydrophobicity of the C4 ligand provides effective retention while helping maintain high recovery of valuable biomolecules during purification.

68004-P21-1503M is a preparative HPLC column with dimensions of 21.2 mm ID x 150 mm length, packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The larger internal diameter accommodates significantly higher sample loading than analytical columns, making it suitable for purification workflows where isolated compounds are collected for further use. The 150 mm length provides increased resolving power compared to shorter preparative columns, making it ideal for scale-up studies, process development, and purification methods requiring enhanced separation. Typical flow rates are approximately 15–40 mL/min depending on mobile phase conditions and system capabilities.

This column is specifically designed for preparative purification of proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The combination of wide pore architecture, fully endcapped C4 chemistry, and preparative dimensions provides excellent loading capacity, analyte recovery, and reproducible purification performance. A specialized preparative HPLC system capable of higher flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.

Key Benefits

• RP C4 Butyl Wide Pore stationary phase

• 300 Å pore size optimized for biomolecules

• Fully double endcapped chemistry

• Traditional butyl (C4) bonded phase

• Designed for preparative HPLC purification

• Excellent recovery of proteins and peptides

• High sample loading capacity

• 10 µm particles for lower operating backpressure

• Suitable for higher flow rates

• Enhanced resolution with 150 mm column length

Typical Applications

• Preparative HPLC purification

• Protein purification

• Peptide purification

• Enzyme purification

• Biomolecule isolation

• Biopharmaceutical research

• Biotechnology research

• Process development

• Scale-up studies

• Fraction collection

Specifications

• Product Type: Preparative HPLC Column

• Stationary Phase: RP C4 Butyl Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double Endcapped

• Particle Size: 10 µm

• Pore Size: 300 Å

• Column Dimensions: 21.2 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 15–40 mL/min

• Brand: Cogent

Special Order Item Information

• Please contact Customer Service for formal quotation, lead time, and ordering assistance.

• Learn more about Special Order Items and policies.

Important Notes

• Designed for purification rather than routine analytical HPLC

• Intended for collection and isolation of purified biomolecules

• Optimized for proteins, peptides, enzymes, and other large molecules

• Provides substantially higher sample loading capacity than analytical columns

• Requires a preparative HPLC system capable of higher flow rates and fraction collection

• Wide pore structure improves accessibility and recovery of biomolecules

• Special Order Item